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Western Blot ProtocolProtocolThis protocol outlines the reagents, buffer and step-by-step procedure to be used with western blot experimentation. This outlines the steps for electrophoresis in SDS-polyacrylamide gel and transfer to nitrocellulose.
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Immunoprecipitation ProtocolProtocolThis protocol outlines the reagents, buffer and step-by-step procedure to be used with immunoprecipitation experimentation. To be used with immunoblotting and western blotting research methods
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Volume-to-Volume DilutionsProtocolVolume-to-volume dilutions describe the ratio of a solute to the final volume of the diluted solution. A majority of the time, antibody manufacturers suggest a certain starting dilution of antibody to use for a specific application.
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Serial DilutionsProtocolA serial dilution is a step-wise and geometric series of dilutions which starts with a small amount of starting material and amplifies the dilution factor serially by using diluted material as a source for subsequent dilutions.
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Direct ELISA ProtocolProtocolThis protocol outlines the procedure for performing direct ELISA methodology. Infomration includes required reagents, preparation of reagents and the procedure for all steps involved.
