Protocols
-
Preparation of Cell Lysate used for ImmunoprecipitationProtocolSee the protocol outlining the preparation of cell lysate used in immunoprecipitation, with the required reagents and the step-by-step procedure.
-
BioReady 40 nm NHS Gold Covalent Conjugation ProtocolProtocolNanoComposix BioReady™ 40 nm NHS Gold can be covalently conjugated to primary amines (-NH2) in proteins using a simplified format. Covalent coupling of proteins (e.g. antibodies) to the gold nanoparticle surface yields robust and reliable gold conjugates. The surface of BioReady™ 40 nm NHS Gold nanoparticles is functionalized with an active NHS ester, allowing amide bonds to form directly between the activated gold nanoparticle and your antibody. This eliminates the need to perform multi-step EDC/NHS chemistry normally required for covalent conjugation, significantly reducing the time and processing required for conjugation.
-
BioReady Streptavidin Gold Conjugation ProtocolProtocolNanoComposix BioReady™ Streptavidin Gold can be conjugated to biotinylated proteins through affinity interactions. The avidin-biotin interaction is one of the strongest non-covalent affinity interactions. BioReady™ gold nanoparticles with Streptavidin are prepared by covalently attaching the tetrameric protein to the surface of the functionalized nanoparticles, with excellent retention of biotin-binding activity.
-
BioReady 80 nm Bare Gold Passive Conjugation ProtocolProtocolThe following procedure demonstrates the process of pH titration to conjugate an IgG antibody to 250 µL of OD 20 BioReady™ 80 nm Bare Gold. A pH range at 7, 8, and 9 will be evaluated to determine the optimal point for passive binding. If desired, 0.5 or 0.2 (or any other increment) of pH point can also be investigated. A larger range of pH from 6–10 can also be evaluated if necessary with the appropriate buffers (see Further Optimization).
-
BioReady 150 nm NHS Gold Nanoshells Covalent Conjugation ProtocolProtocolNanoComposix BioReady™ 150 nm NHS Gold Nanoshells can be covalently conjugated to primary amines (-NH2) in proteins using a simplified format. Covalent coupling of proteins (e.g. antibodies) to the gold nanoparticle surface yields robust and reliable gold conjugates. The surface of BioReady™ 150 nm NHS Gold Nanoshells are functionalized with an active NHS ester, allowing amide bonds to form directly between the activated gold nanoparticle and your antibody. This eliminates the need to perform multi-step EDC/NHS chemistry normally required for covalent conjugation, significantly reducing the time and processing required for conjugation.
Bulk Request
Request bulk quantities of our high quality antibodies to enable your large-scale projects.
Request a quote and share items with your colleagues or purchasing team by adding to a shareable list.
