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How to Optimize Your Multiplex Testing for Diagnostics ApplicationsArticleOriginal article written and published by Today’s Clinical Lab
Custom reagents for multiplex PCR assays may be the missing ingredients for your infectious disease panel -
USP5 promotes breast cancer proliferation and metastasis by stabilizing HIF2αArticleHypoxia is often present in solid tumors as cell growth and density increases limiting oxygen diffusion at normal levels to all the cells. While hypoxia and hypoxia inducible factors have been associated with metastasis and poor clinical prognosis, not much has been studied about the regulation of HIF2α in breast cancer. This study identifies USP5 as a novel deubiquitinase for HIF2α, increasing HIF2α stability and transcriptional activity. Together, these proteins increase cell proliferation and metastasis, and their expression positively predicts poor patient outcomes.
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Cooperative interaction between ERα and the EMT-inducer ZEB1 reprograms breast cancer cells for bone metastasisArticleEpithelial to mesenchymal transition (EMT) is a hallmark of cancer progression and in breast cancer not only predicts poor prognosis, but also resistance to endocrine therapy. A recent paper outlines the interactions between ZEB1 and estrogen receptor alpha and identifies an EMT hybrid population that could be targeted in novel therapeutic strategies to reverse EMT and improve patient prognosis.
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Solutions for Common Challenges in Developing PCR-Based Diagnostic AssaysArticleMolecular diagnostic (MDx) assays are used to detect DNA/RNA in human samples and are essential in the clinic to detect and prevent diseases. MDx assays based on the polymerase chain reaction (PCR) are easy-to-use and can deliver results fast and with great sensitivity. However, researchers developing PCR-based assays can encounter several hurdles along the way. This application focus highlights solutions for common challenges faced during the development of PCR-based assays for molecular diagnostics applications.
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The MuvB complex binds and stabilizes nucleosomes downstream of the transcription start site of cell-cycle dependent genesArticleThe MuvB complex is a critical regulator of cell cycle gene transcription. Alterations in binding partners at different stages of the cell cycle activate or repress gene transcription. This study found that MuvB transcriptional repression is dependent on nucleosome binding in the 1+ position.
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